Delineating the transcriptional network of prognostic gene signatures refines treatment recommendations for lymph node negative breast cancer patients

To define proliferative and senescent cell transcriptional profiles, we performed genome-wide expression analyses of growing and senescent mouse embryonic fibroblasts (MEFs) MEFs were passaged from P1 (growing) to P7 (replicative senescence) and RNA was harvested from 3 independent MEF lines at P2 and P7. This RNA was used for genome-wide expression analyses.