Accipiter nisus potential microsatellite loci

Full library of potential microsatellite loci for Accipiter nisus.

Based
on DNA from tissue samples of four specimens of A. nisus from Tenerife,
Germany, Finland and Georgia. DNA digestion with restriction enzymes
AluI, RsaI, and Hpy166II. Blunt ends were adenylated (+A) with Klenow
(exo-) and dATP, and after heat inactivation of the Klenow (exo-), the
reactions were supplemented with ATP to 1 mM and an Illumina Y-adaptor
was ligated with T4 DNA ligase. Enrichment of the fragments for
microsatellites by hybridisation to and magnetic capture of biotinylated
repeat probes (representing two unique dimers, five unique trimers,
seven unique tetramers and two unique pentamers), followed by
amplification and barcoding by PCR, and sequencing on an Illumina MiSeq
instrument (2 × 250 bp paired reads). SeqMan NGen (version 11) was used
for raw read assembly, and the program msatcommander 1.0.8_beta (for Mac
OSX) was employed to scan the assembly for microsatellite loci and
automatically design primer pairs.