Single Cell Analysis of Proteoforms

We introduce single cell Proteoform imaging Mass Spectrometry
(scPiMS), which realizes the benefit of direct solvent
extraction
and MS detection of intact proteins from single cells dropcast onto
glass slides. Sampling and detection of whole proteoforms by individual
ion mass spectrometry enable a scalable approach to single cell proteomics.
This new scPiMS platform addresses the throughput
bottleneck in single cell proteomics and boosts the cell processing
rate by several fold while accessing protein composition with higher
coverage.