The arf10/16 double mutant has a differential transcriptional program in response to Al stress. Arabidopsis thaliana

To reveal how ARF10 and ARF16 regulate Al-induced root-growth inhibition, a transcriptome analysis was carried out by comparing without and with Al-exposed arf10/16 double mutant line and WT through RNA sequencing. The present transcriptomic analysis has revealed that many of the differentially transcribed genes associated with cell wall modification were regulated by transcription factors ARF10 and ARF16. The implication is that the auxin-regulated Al-induced inhibition of root growth arises from auxin signalling-regulated cell wall structure or component modification. Overall design: Total RNA obtained from control (non-treated roots) and Al-exposed roots. Six day old plants were treated with and without Al treatment for 24 h, then total RNA was isolated using the RNeasy® Plant Mini Kit (QIAGEN), and then Illumina sequencing was performed.