Schematic representation of perfusion setup for gastric acid secretion experiment in suncus.

After anesthesia, a suncus was placed on a culture flask containing circulating hot water from a water bath. A logger was used to monitor body temperature during the experiment. Perfusion of saline solution was done by a peristaltic pump, and gastric output was collected through a catheter from the stomach at intervals of 10 minutes. Effluents were used to measure pH and acid output. Intravenous drug administration was done through the jugular vein. (EPS)