Kinetic parameters for amyloid formation from metalated SOD1 at pH 7 in the presence or absence of TCEP.

SOD1 was incubated in 50 mM MOPS, 0.1 M NaCl, pH 7 with or without TCEP. The time course of ThT fluorescence was fit to a sigmoidal equation and the amplitude and lag time determined as described in “Materials and Methods”. Dashes indicate that no amyloid was formed. Location: BB = beta barrel; DI = dimer interface; MBR = metal-binding region. Cu and Zn contents are metal atoms per dimer.