Self-organizing in vitro mouse neural tube organoids mimic embryonic development

Self-elongating neural tube organoids recapitulate key aspects of the morphology, anterior-posterior patterning, neural crest emergence and neural differentiation of mouse embryo in vivo by self-organization. We used single-cell RNA sequencing (scRNA-seq) to analyse the cell types and to reveal the sequence of transcriptional events in the emergence of neural crest cells and neural differentiation. 10x single-cell RNA-seq of mouse neural tube organoids at day 6 (in duplicates), 7, 8, and 10.