Epigenome analysis of angiogenin impact on bladder cancer

We investigated if Overexpression of ANG in UROtsaANG and RT112Scr could cause epigenetic changes, comparing to UROtsaEmpty and RT112KD-ANG, respectively . The alteration of genome-wide DNA methylation in these cells was analyzed using Illumina whole genome methylation array. Specifically, methylated DNAs with 5-mC were amplified using nonbiased conditions, labeled and hybridized to a BeadChip array covering over >450,000 methylation sites. ANG expression was noted to be associated with a change in global DNA methylation levels compared to control cells Bisulphite converted DNA from the 4 samples were hybridised to the Illumina Infinium HumanMethylation450 BeadChip. The bisulfite treatment, microarray service and analysis of the array were provided by Macrogen Inc. (Seoul, South Korea).