Enzyme kinetic parameters of three GSTE2 alleles with substrate DDT.
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Three variant GSTE2 proteins were expressed from a DDT resistant (ZAN/U) and susceptible (Kisumu) strain of An. gambiae and assayed with substrate DDT over a range of concentrations. The maximum enzyme rate (Vmax), substrate concentration at half the maximum rate (KM) and catalytic turn-over (Kcat) were calculated for each protein from a Michaelis-Menten or substrate inhibition equation (Figure 1).
创建时间：
2015-12-02
