Cellular diversity of the regenerating caudal fin

single cell transcriptomics of preinjury and regenerating caudal fin tissue revealed cell type consistency and cell-type-specific genetic program during regeneration Overall design: Preinjury and 1/2/4 day post amputation caudal fin tissues were collected and digested by Accumax (Innovative Cell Technologies), filtered through 40-µm cell strainers, and washed with 1× Dulbecco's phosphate-buffered saline (DPBS)–0.04% bovine serum albumin to generate single-cell suspensions. Libraries were constructed from these cell suspensions following the instruction of the Chromium Single Cell Gene Expression Solution 3' v2 (10x Genomics) and were subsequently sequenced on HiSeq2500 (Illumina) with read lengths of 26 + 75 (Read1 + Read2). Raw reads were processed by Cell Ranger (10x Genomics) with default parameters for read tagging, alignment to zebrafish reference genome (GRCz10), and feature counting based on Ensembl release 91 (cellranger count).