nanopore_directRNA-seq

Respiratory viruses including influenza A virus continue to cause annual epidemics with high morbidity and mortality due to limited effectiveness of existing vaccines and antiviral drugs. Among the strategies evolved by viruses to evade immune responses is host shutoff - a general blockade of host messenger RNA and protein synthesis. Influenza A virus encodes two proteins that function in the host shutoff: NS1 and PA-X. To determine if NS1 function has a direct effect on PA-X activity by analysing host shutoff in human epithelial cells infected with wild type or mutant IAVs with PA-X and NS1 effector domain deletion, we used direct RNA sequencing by oxford nanopore technology.The fastq files were generated after guppy basecalling.