Positive controls, feeding trials, and negative controls.

Positive controls (rows 1–3 in the table) were designed to estimate the number of sequences recovered as a function of the quantity of C. sordidus material assayed, which was varied by processing different numbers of C. sordidus eggs. The feeding trial (rows 4–6 in the table) was designed to determine whether C. sordidus DNA could be detected in the gut contents of a predator (O. baurii in this case) that had consumed one C. sordidus egg (in related treatments, the predator had not consumed a C. sordidus egg or was processed with one egg that it had not consumed). Negative controls (rows 7 and 8 in the table) consisted of ultra-pure HPLC grade water.