Gene expression profile at single nuclei level of cells from mouse hippocampus

We performed single-nucleus RNA-seq and chromatin accessibility analyses (snRNA-seq and ATAC-seq) in the hippocampus of wild-type (WT) mice and mice carrying amyloid precursor protein knock-in mutations (APPNL-G-F) in response to chronic wheel-running exercise. Overall design: 8-week old female WT and APPNL-G-F mice were put into cages with running wheel for voluntary exercise for 10 weeks. Mice from 4 different conditions (WT_RT, WT_EX, APP_RT, APP_EX) were subjected to single nuclei RNA and ATAC sequencing.