Extrinsic KRAS signaling shapes the pancreatic microenvironment through fibroblast reprogramming

We utilized the inducible and reversible iKras* genetically engineered mouse model to intiate oncogenic Kras (Kras*) expression in the pancreatic epithelium by providing mice with doxycycline water, and then switched mice to regular water thereby turning expression of Kras* off for 3 days, 3 weeks post-induction of acute pancreatitis. Single cell RNA sequencing revealed that fibroblasts are reprogrammed by signals originating from epithelial Kras*-expressing cells to shape the immune microenvironment during pancreatic tumorigenesis. This 10X Genomics single cell RNA sequencing repository includes raw and processed data files from iKras* mice expressing oncogenic Kras (Kras*) for 3 weeks following induction of acute pancreatitis, and then without Kras* expression for an additional 3 days prior to harvest (n=3 mice pooled prior to submission). Single cell cDNA libraries were prepared and sequenced at the University of Michigan Advanced Genomics Core using the 10x Genomics platform. The sample was run using paired-end 50 cycle reads on HiSeq 400 (Illumina) to a depth of 100,000 reads. Alignment was performed by the University of Michigan Advanced Genomics Core. Parameters include Cell Ranger version 3.0.0 (default settings), Single Cell 3' v3 chemistry, mm10 transcriptome, initial expected cell count of 10,000.