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Control of macrophage response to TNF by the sst1 locus

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE164698
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We studied effects of the mouse sst1 locus that controls susceptibility to intracellular bacteria, on macrophage response to TNF. Bone marrow-derived macrophages were isolated from the B6 wild type and the sst1 congenic B.Sst1S mice and differentiated in vitro in the presence of CSF1. Cells were stimulated with 10 ng/ml of TNF for 12 hours prior to RNA isolation.
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2023-12-31
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