oyster hemolymph transcriptomes challenged with Perkinsus marinus and apoptosis modulators

Pooled eastern oyster hemolymph from a mixture of selectively bred families (VIMS) was challenged in triplicate with filtered sterile seawater (FSSW) (control), Perkinsus marinus (1:1 MOI with hemocyte concentration) for 1hr, P. marinus (1:1) for 1hr following a 3hr incubation with the IAP inhibitor GDC-0152, or P. marinus (1:1) for 1hr following a 1hr incubation with the caspase inhibitor Z-VAD-FMK. Samples were flash frozen with liquid nitrogen after 1hr of challenge and stored at -80C prior to RNA extraction. RNA was extracted from hemocyte samples using a Trizol protocol and sequenced at a depth of 15-20 million reads per sample (12 samples total).