Software for Quantification of the NA dependent change of shape in the image ...

The point spread function of a fixed fluorophore with its dipole axis colinear to the optical axis appears donut-shaped when seen through a microscope, but can change to a spot...当通过显微镜观测时，偶极轴与光轴共线的固定荧光团的点扩散函数（point spread function）呈现甜甜圈形形貌，但其可转变为光斑……