FoxP3 mRNA-seq in mouse CD4+T cells

To investigate the impact of FoxP3 multimerization mutants on FoxP3's genome-wide gene expression profiles, we conducted FoxP3 RNA-seq experiments using mouse CD4+ T cells. The results indicated that all of these multimerization mutants had an effect on FoxP3 gene expression profiles, including some well-established Treg signatures. Overall design: mRNA-seq on Wild-type FoxP3 overexpressed in mouse CD4+ T cells, alongside an empty vector control and four distinct multimerization mutants. Each sample was quadruplicated for robustness.