Valine and non-essential amino acids affect bi-directional transport rates of leucine and isoleucine in bovine mammary epithelial cells

“Valine and non-essential amino acids affect bi-directional transport rates of leucine and isoleucine in bovine mammary epithelial cells”. This study assessed transport rates of 7 AA to evaluate the effects of high and low concentrations of Val and Ala, Gln, and Gly provided as a group in primary bovine mammary epithelial cells. Treatment effects on Leu and Ile were attributed to increased intracellular concentrations of non-essential AA used as exchange currency combined with the reduced shared transporter competition from Val. These results demonstrate that the efficiency of branched-chain amino acid uptake by mammary cells is affected by competition among the branched-chain AA and the synergistic effects of non-essential AA.

本研究探讨了牛乳腺上皮细胞中，氨酸与非必需氨基酸对亮氨酸和异亮氨酸双向转运速率的影响。研究评估了7种氨基酸的转运速率，以评估高浓度和低浓度的氨酸、丙氨酸、谷氨酰胺和甘氨酸组合对初级牛乳腺上皮细胞的影响。对亮氨酸和异亮氨酸的处理效果归因于非必需氨基酸作为交换货币的细胞内浓度增加，以及氨酸减少的共享转运蛋白竞争。这些结果揭示了乳腺细胞对支链氨基酸摄取效率受到支链氨基酸之间的竞争作用及非必需氨基酸的协同效应所影响。