five

The effect of talc on J774 cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE135238
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The experiment tests the hypothesis that talc particles affect the trasncriptome of phagocytosing cells. Macrophages were plated and exposed to 10 µg/well of particles (talc (T) or titanium dioxide (Ti)) and 2 µg/mL of 17-β estradiol (E) or vehicle. After 24 hours of particle and estrogen treatment, the RNA was extracted through a RNeasy Mini Kit (Qiagen).
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2019-09-18
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