Single cell transcriptomic profile of splenic B and T cells from B-cell specific Il2rb deficient mice

IL-2 is one of the first cytokine discovered but its complex roles in T cell effector functions has shadowed its function in B cell responses. B cells transiently expressed IL-2 receptor upon activation but it is unclear whether all mature B cell subsets have an equal dependence upon IL-2 and how IL-2 dictates B cell fate. Using our newly generated B-cell-specific Il2rb conditional knockout (KO) model of mice lacking IL2RB signaling in mature B cells, our objectives were to characterize the heterogeneity of splenic B cell early response to in vivo immunization (SRBC) comparing scRNAseq data from control and KO mice. In parallel, we analyzed T cell subsets. Overall design: Il2rb KO and Ctrl mice were immuninized intraperitoneally with sheep red blood cells (SRBC). Spleen of unimmunized mice (KO and Ctrl) and day 4 SRBC-immunized mice (KO and Ctrl) were recovered. Splenic B and CD4+ T cells were magnetically isolated. We then extracted RNA and performed scRNA-sequencing. ADT/HTO: barcode sequence: hashtagged sample TotalSeq-C0301 anti-mouse Hashtag1: ACCCACCAGTAAGAC: KO D4 TotalSeq-C0302 anti-mouse Hashtag2: GGTCGAGAGCATTCA: Cre D4 TotalSeq-C0303 anti-mouse Hashtag3: CTTGCCGCATGTCAT: KO D0 TotalSeq-C0304 anti-mouse Hashtag4: AAAGCATTCTTCACG: Cre D0