Sensitivity of SIBA extension to point mutations.

The SIBA reaction was performed with either a fully homologous target template (SB-template) or with templates containing 1–4 base point mutation(s). The results are expressed as the delay in the threshold detection time (dt), i.e., the average Δdt = average dt of a template containing point mutation(s) minus the average dt of the fully homologous target template (SB-template). ND denotes no detectable amplification of a template. SB-F21 and SB-R21 were the upstream and downstream primers, respectively. The invasion oligonucleotide (IO) used was SB-IO. Sensitivity of SIBA extension to point mutations.