Gene expression analysis at single cell level from the CNS of mice that harbored Melanoma and recieved Anti-PD1 antibody or Isotype control antibody treatment

Immue checkpoint blockade (ICB) has significantly improved the overall survival of melanoma patients. Despite high success rates, patients often experience side effects that are collectively termed as immune related adverse events that potentially target any organ including the CNS. However, the role of microglia in ICB-induced neurotoxicity was unclear. We studied the impact of microglial activation in response to ICB. ScRNA sequencing was employed to decipher diverse microglia populations and other cell types from the cortex. Melanoma cells were intravenously injected. Anti PD1 or Isotype control antibodies was given intra peritoninally at a concentration of 250µg/ dose on the following days - Day1,4,8,16,22. The mice were scarificed on day 23 and brains were isolated. Cell populations from the cortex of the mice were isolated and enriched for microglia by flourescent activated nuclear sorting (FACS) and analyzed using sn RNA sequencing