Data associated with "Utilizing a novel fecal sampling method to examine resistance of the honey bee (<i>Apis mellifera</i>) gut microbiome to a low dose of tetracycline."

These are bacterial 16S rRNA gene amplicon data and metadata from honey bee feces and guts; the dataset includes amplicon sequences, sequencing information necessary for demultiplexing and using the amplicon data, and tetracycline resistance gene (tetB and tetM) qPCR data for a subset of the samples. This study comprised two parts. The first was a test to confirm that fecal samples from honey bees were a useful method to assess the bacterial gut community of honey bees. The second was a lab study using the fecal sampling method to investigate the impact of a low dose of tetracycline on resistance of the honey bee gut microbiome to disruption by antibiotics, focusing on responses of individual bees.