Transcriptomic and metabolomic data of Populus deltoides ‘Shalinyang’ (PdS) response to feeding of Anoplophora glabripennis adults

Populus deltoides ‘Shalinyang’ (PdS), a vital species for artificial shelterbelts in the arid regions of northwest China, has shown resistance to the destructive stem-boring beetle Anoplophora glabripennis Motschulsky (Asian longhorned beetle, ALB). However, comprehensive research in this area is still lacking. This study compared PDS leaves and phloem tissues before and after ALB adult feeding, utilizing untargeted metabolomics (LC-MS/MS) and transcriptomics (RNA-Seq) methods. The investigation identified a total of 3,552 metabolites across the 12 samples. Transcriptomic sequencing generated 82.52 Gb of clean data, with an average of 6.88 Gb per sample, and annotated 33,429 expressed genes. These data provide fundamental and valuable information for understanding how the resistant species PdS responds to ALB adult infestation. Furthermore, they can be utilized in future studies to explore the interaction mechanisms between poplar species and ALB and investigate the insect resistance mechanisms of PdS.