aAll the data in this table are derived from ITC experiments conducted in 20 mM [Na+] citrate-phosphate buffer, pH 7.0 and are average of four determinations, Ka and ΔHo values were determined from IT
The kinetic parameters, association rate constants (kon) and dissociation rate constants (koff), were determined using the BIAcoreX system for SPR detection. Biotinylated peptide bL15Vp (600 resonance
Kinetic constants of interaction between GFP-ready-TNFα and GFP variants, determined by interferometry. kon, the association rate constant, in M-1s-1. koff, the dissociation rate constant in s-1. KD,
