Quantitative Analysis of erythroblast cells Transcriptomes treated with Carbon Dots(CDs) derived from Codonopsis pilosula or not

In this study, distinctive biological CDs derived from Codonopsis pilosula were designed and synthesized. In traditional Chinese medicine, Codonopsis pilosula is considered to be a good blood tonic. The synthesized CDs comprise sp2/sp3 carbon atoms and oxygen/nitrogen-based groups and exhibit good biocompatibility and membrane permeability. The in vitro and in vivo experiments were conducted using an erythroid differentiation system and a mouse model, respectively, and showed that the CDs significantly promote the proliferation of erythroid cells. To reveal the detailed mechanisms of CDs in stimulating erythroblast proliferation, we performed RNA sequencing to analyze the gene expression profile change between untreated andmCDs treated erythroblasts. Overall design: TER119-negative fetal liver erythroblasts were purified and cultured in Epo containing medium for 30 h supplemented with or without CDs (1 ug/ml), and total RNA was extracted using the TRIzol reagent. RNA sequencing was conducted using the BGIseq500 platform (Shenzhen, China) in triplicate.