Information S1 - Species-Selective Killing of Bacteria by Antimicrobial Peptide-PNAs

Figure S1. Venn diagram of the fifteen possible species combinations (lettered A–O) in a theoretical bacterial community composed of the four species used in this study. The key on the right indicates the antimicrobial agent that would result in the bactericide of the desired species target(s). Peptide-PNAs are in bold. Only the peptide-PNAs designed in this study are capable of species-specific bactericide for individual (C and D) and mixed (M) Gram-negative species. * Peptide-PNA (Ec1000) was non-specific, but a number of E. coli- specific PNAs were designed (Table S4–6 in Information S1) that have yet to be evaluated. Figure S2. The predicted structures of the E. coli PNA transporter protein SbmA (pink) and YgaD (blue) of B. subtilis. Structures were predicted using the I-TASSER platform [1]. YgaD was identified by homology searching using HHPred [2] with SbmA as the input sequence. Protein sequences were aligned and rendered by PyMOL [3]. Table S1. Antibiotic susceptibility of strains used in this study. Table S2. S. Typhimurium-specific PNAs. Table S3. S. Typhimurium-specific PNAs designed using essential genes from E. coli. Table S4. E. coli-specific PNAs. Table S5. Number of cells used for inoculation of mixed culture. Table S6. Oligonucleotides used in this study. (DOCX)