Transcriptomic changes in the colon of WT and NAPRT KO mice after AOM injection [NAPRTKOAOM]

NAPRT-mediated deamidated biosynthesis of nicotinamide adenine dinucleotide (NAD), an essential electron carrier for hundreds of biochemical reactions in all living cells, mediates the diet-microbe-host NAD metabolic interaction in mice. To study the function of this metabolic pathway in vivo, we recently generated a mutant mouse strain (NAPRT KO) in which a 21-nt fragment that encodes 7 amino acids in the catalytic domain of NAPRT was deleted in all cells using the CRISPR/Cas9-mediated gene editing technology. Our results showed that NAPRT is highly expressed in the gut epithelial cells, where it functions to maintain the cellular NAD pool for an efficient response to stress-induced acute NAD depletion. Consistently, NAPRT deficiency impairs DNA repair, sensitizes mice to AOM-induced gut DNA damage. Here we profiled transcriptomic changes in the colon of WT and NAPRT KO mice at different time points after injection of AOM. Overall design: Female WT and NAPRT KO mice supplemented with nicotinic acid at 3 g/L in drinking water were i.p. injected with 10 mg/Kg AOM, colons were then harvested at 0 (control) , 12, 48 hr after AOM injection (n=7-9 mice/genotype in each tme point)