Crassostrea gigas hemocytes scRNA-seq sequences. hemofight

The 10X Genomics protocol was followed to prepare gel in emulsion beads containing single cells, hydrogel beads, and reverse transcription reagents, perform barcoded cDNA synthesis, and generate sequencing libraries from pooled cDNAs. The concentration of single cell suspension was approximately 1000 cells / L, and cells were loaded according to the 10X protocol to capture approximately 3000 cells per reaction. Library construction was performed using 10X reagents according to the manufacturer. Libraries (paired end reads 75 bp) were sequenced on an Illumina NovaSeq using two sequencing lane per sample.