SNP genotyping in HS mice.

All polymorphisms were analysed by allele discrimination using a 7500 Fast real time PCR system (Applied Biosystems). For probe details see Table S2 in File S1. For all genotypes, the statistical test used was the Kruksal-Wallis non-parametric ANOVA. The allelic test used was the Mann-Whitney test. dbSNP identifiers are provided with the polymorphism descriptions. Approximately 400 samples were genotyped for each SNP however, the precise number for which genotypes were collected varied due to random technical failure.