RNA-Sequencing reveals mechanisms by which human glial progenitor cells (hGPCs) initiate remyelination

To investigate transcriptomic drivers of human myelination, immunodeficient mice were chimerized with fetal derived hGPCs at P1, sacrified at 36 weeks, and hGPCs were extracted for RNA-Sequencing via FACS. Mice were either fed a control diet for the entireity of their lives or a demyelinating diet beginning at 12 weeks of age until 24 weeks, when they were transitioned to a control diet to allow for remyelination to begin. RNA-Sequencing of FACS isolated hGPCs from the corpora callosa of 36 week old mice fed a control diet (n=6) or following 12 weeks of a demyelinating cuprizone diet beginning at 12 weeks, for 12 weeks, before being allowed to recover on a control diet for 12 weeks (n=6).