Investigating_the_role_of_bacterial_genetic_variation_in_syphilis_disease_progression_using_whole_genome_sequencing

To date, the majority of Treponema genomics studies have been performed on opportunistically collected patient samples, with limited clinical information and patient connectivity metadata. Syphilis is characterised by active diseases stages (primary, secondary, tertiary), separated by periods of latency that can span months to decades. The three disease stages are classically described as quite distinct clinical conditions, with different symptoms and sequelae. The TREPOLI study conducted in Amsterdam (Netherlands), has been prospectively following and collecting samples from MSM patients with an infectious syphilis infection (primary, secondary or early latent stage), including collecting multiple samples per patient at different body sites. This collection presents an unusual opportunity to explore the genomics of syphilis disease progression within a high prevalence population, and we will sequence T pallidum genomes from patient samples to assess the evidence for convergent evolution associated with disease progression.