Data from: Weights of hatchlings across different temperature treatments

Global warming is one of the most significant and widespread effects of
climate change. While early life stages are particularly vulnerable to
increasing temperatures, little is known about the molecular processes
that underpin their capacity to adapt to temperature change during early
development. Using a quantitative proteomics approach, we investigated the
effects of thermal stress on octopus embryos. We exposed Octopus berrima
embryos to different temperature treatments (control 19 °C, current summer
temperature 22 °C, or future projected summer temperature 25 °C) until
hatching. By comparing their protein expression levels, we found that
future projected temperatures significantly reduced levels of key eye
proteins such as S-crystallin and retinol dehydrogenase 12, suggesting the
embryonic octopuses had impaired vision at elevated temperature. We also
found that this was coupled with a cellular stress response that included
a significant elevation of proteins involved in molecular chaperoning and
redox regulation. Energy resources were also redirected away from
non-essential processes such as growth and digestion. These findings,
taken together with the high embryonic mortality observed under the
highest temperature, identify critical physiological functions of
embryonic octopuses that may be impaired under future warming conditions.
Our findings demonstrate the severity of the thermal impacts on the early
life stages of octopuses as demonstrated by quantitative proteome changes
that affect vision, protein chaperoning, redox regulation, and energy
metabolism as critical physiological functions that underlie the responses
to thermal stress.