Whole genome shotgun survey sequencing of sorted Rye Chromosomes

Rye (Secale cereale L.) (2n = 2R = 14) is an important genetic resource in wheat and triticale breeding because of its abiotic stress tolerance, self-incompatibility, and extensive heterosis. In order to unlock the genetic potential of rye, a high density consensus transcript map comprising 3,588 rye EST-SNP and EST-SSR markers and representing 2,886 genes was constructed. Furthermore, survey sequences of all 7 rye chromosomes were generated and the gene content information was integrated on the basis of the dense transcript map and conserved synteny information of sequenced grass genomes into virtual gene order maps of all seven rye chromosomes comprising over 20,000 genes.