RNAseq anaysis of hippocamal GAD2 cells
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Purpose: To identify gene expression in GAD2 neurons of the hippocsmpus Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from GAD2+ cells, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: GAD2 had ~8000 of enriced genes as compared to bulk mRNA Overall design: 2 hippocampi were pooled for one translating ribosome affinity purification sample. We used mice that were GAD2 cre+ crossed with L10-EGFP+ mice.
创建时间：
2023-02-11
