Lipidomic datasets for: Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism

Transmembrane protein 135 (TMEM135) is thought to participate in the cellular response to increased intracellular lipids yet no defined molecular function for TMEM135 in lipid metabolism has been identified. In this study, we performed a lipid analysis of tissues from Tmem135 mutant mice and found striking reductions of docosahexaenoic acid (DHA) across all Tmem135 mutant tissues, indicating a role of TMEM135 in the production of DHA. Since all enzymes required for DHA synthesis remain intact in Tmem135 mutant mice, we hypothesized that TMEM135 is involved in the export of DHA from peroxisomes. The Tmem135 mutation likely leads to the retention of DHA in peroxisomes, causing DHA to be degraded within peroxisomes by their beta-oxidation machinery. This may lead to generation or alteration of ligands required for the activation of peroxisome proliferator-activated receptor a (PPARa) signaling, which in turn could result in increased peroxisomal number and beta-oxidation enzymes observed i..., Dataset was collected by the following methods:
Sample preparation for lipidomics analysis. Livers, retinas (neural retinas and eyecups), hearts, and plasmas were collected and stored at -80°C prior to lipid extraction. Prior to extraction, all solutions were pre-chilled on ice. Samples were transported and cut on a dry ice-cooled stainless steel plate and weighed on a Mettler (XSR205) analytical balance to the nearest hundredth of a milligram. This weight would be used to normalize samples for analysis. Fifty microliter aliquots of plasma from each mouse were used in this study. No normalization occurred with the plasma samples since the same volume for each sample was used in this study. Tissues were then placed into Qiagen PowerBead tubes (P/N 13113-50) for homogenization and extraction. Lipids were extracted in a solution of 250 µL PBS, 225 µL methanol containing internal standards (Avanti SPLASH LipidoMix (Lot#3307-07) at 10 µL per sample) and 750 µL MTBE (methyl tert-butyl ether)...., The programs required to open the data files include Microsoft Excel and MetaboAnalyst 5.0 (https://www.metaboanalyst.ca).Â