Quantification of the PTZ levels in the brain.

The concentration of PTZ in the brain was determined as μg PTZ/mL sample at 5 mM (gray bars) with values of 1.885, 3.175, 1.612, and 10 mM (blue bars) with values of 3.222, 7.905, and 2.870, after 2.5 and 20-min of PTZ exposure, and after 60-min washout. Data are expressed as mean ± S.E.M and analyzed by two-way ANOVA followed by Bonferroni’s test as post-hoc. Distinct letters indicate statistical difference within the same group at different periods, (lower case letter for 5 mM and capital letters for 10 mM) whereas the asterisks (*) indicates significant difference between both PTZ groups for each time.