transcript analysis of strains with or without plasmid containing 40 genes in three different culture conditions

Purpose: To investigate the influence of the 40 genes on plasmid, we compared the transcript varies between the engineered strains and the wild-type strain in synthetic medium with different carbon sources (i.e., glucose, galactose and ethanol/glycerol media). Methods: Total mRNA profiles of the engineered strains and the wild-type strain were generated by deep sequencing, in triplicate, using BGIseq500. The sequence reads that passed quality filters were analyzed. Results: RNA sequencing results revealed that the transcript patterns were influenced dramatically by the carbon sources and there was no significant difference between the wild-type and engineered strains (that is, any change amounted to less than 10%) Overall design: Total mRNA profiles of the engineered strains and the wild-type strain which grew to log phase and collected.