Human Preadipocyte Response to PCB52 or 4-OH-PCB52

Purpose: The goal of this study was to perform an expression transcript profile over a timecourse of human preadipocytes that were treated with PCB52 or 4-OH-PCB52; Methods: Immortal human preadipocytes (NPAD) were treated with 10 uM PCB52 or 4-OH-PCB52 in PGM2 media containing 10%FBS for 9 hrs, 1 day, or 3 days. RNA was extracted with TriZol, DNase treated, and purified using a Qiagen column. RNAseq was used to assess gene expression. Bioinformatic analyses were used to determine differential gene expression between PCB52 or 4-OH-PCB52 treated and vehicle-treated at the same timepoint. Overall design: RNAseq profile of differential gene expression over a timecourse caused by treatment of human preadipocytes with PCB52 or 4-OH-PCB52