File S1 - p62/SQSTM1 Accumulation in Squamous Cell Carcinoma of Head and Neck Predicts Sensitivity to Phosphatidylinositol 3-Kinase Pathway Inhibitors

Figure S1. A) MK-2206 and SAR245408 inhibit AKT and S6 phosphorylation. B) MK-2206 and SAR245408 efficiently induce G1-arrest in SCC61 cells but not in SCC35 cells. Figure S2. Effect of MK-2206 and SAR245408 on AKT phosphorylation and cyclin D1 protein in SCCHN cell lines. Figure S3. Alterations of ATG7 gene in the TCGA Squamous Cell Carcinoma of Head and Neck (SCCHN) database and overall survival. Table S1. IC50 of MK-2206 and SAR245408 in SCCHN cell lines. Table S2. Apoptotic assay after 48 hours of treatment with MK02206 or SAR245408. The values are expressed as percentage of total cell number counted. Table S3. Intrinsic autophagy competence determined by LC3-II to LC3-I ratio and IC50 of respective agents in SCCHN cell lines. Table S4. Relationship between p62/SQSTM1 protein expression and IC50 in SCCHN cell lines. Pearson rank correlation was used to calculate correlation. Table S5. Relationship between p62/SQSTM1 protein expression and IC50 in breast cancer cell lines. Pearson rank correlation was used to calculate correlation. Table S6. Primer sequences for ATG7 MSP analysis.
(DOC)