Aflp Dataset And Sequence Alignments From A Study Of Rhododendron Ferrugineum Across The Whole Species Range.

STRUCTURE DATASET The raw data are in the files: Rfe_KAR_AAT-CAC2_2017-10-18-16-55-16.zip Rfe_KAR_ATC-CAC_2017-10-25-12-39-28.zip Rfe_KAR_ATG-CTG2_2017-10-24-09-40-25.zip Population data: populations.txt The final structure file: Rfe.str We analysed the obtained results in GeneMapper (Applied Biosystems) with default settings for AFLP analysis. The combinations of selective starters amplified the following numbers of polymorphic markers: AAT-CAC=127 (mean=40,4; SD=8,1), ATC-CAC=119 (mean=35,3; SD=5,4), ATG-CTG=144 (mean=44,4; SD=5,0). The blank samples yielded 0, 6 and 5 markers, respectively, which were removed from the final data matrix. We also removed the markers present in only one individual. Three samples (one from each of the populations L22, K27 and S05) failed to amplify in one of the selective primer reactions and were thus removed from the final matrix. The estimated error rate was 2.87% and the final matrix comprises of 87 individuals and 254 polymorphic loci. SEQUENCE ALIGNMENTS Rfe_ITS_align.fas Rfe_rpl32-trnL_align.fas Rfe_RPS12-RPL20_align.fas Rfe_trnfM-trnS_align.fas Rfe_trnLF_align.fas