Age-related noncanonical TRMT6–TRMT61A signaling impairs hematopoietic stem cells

We reported the tRNA-m1A sequencing results performed in young and aged hematopoietic stem and progenitor cells. Our results defined aging-associated alterations of tRF in hematopoietic stem and progenitor cells and identifed a subset of tRF as hallmark of HSC aging.RNA-seq data analysis of WT v.s. Trmt6/61a-TG hematopoietic stem cell (lineage-, c-kit+ Sca-1+ CD135-, CD34-), the two population of hematopoietic stem cell (HSC) were purified from the bone marrow of WT and Trmt6/61a-TG mice, revealed that differnent expression of WT v.s. Trmt6/61a-TG hematopoietic stem cell.Results provide insight into the role of TRMT6/61A complex in HSC. Overall design: Total 8 m1A-seq samples were analyzed.There are two treatment (AlkB treatment and control) and two age states (Old and Young), and each state are two replications.The AlkB-treated sequencing samples were also utilized for the analysis of tRFs. Total 4 RNA-seq samples were analyzed.There are two genotypes(WT and Trmt6/61a-TG),and each state are two replications.