Mapping genomic binding sites of Drosophila Hox proteins and Hox cofactors, Extradenticle and Homothorax, in Kc167 cells with transient expression [ChIP-seq_Transient]

We have carried out a systematic analysis of the in vivo genomic binding profiles of all eight Drosophila Hox proteins using transient transfection in Kc167 cells to examine Hox protein targeting. In addition, we have examined Hox protein binding in cells transfected with both Hox and the cofactor, Homothorax. Provision of Homothorax also recruits the cofactor Extradenticle. We have examined the binding of all 8 Drosophila Hox proteins both in the absence and presence of the cofactor Homothorax. We also examine the binding of Homothorax and Extradenticle. For each sample type there are two biological replicates and one input sample.