Primary term human cytotrophoblast, untreated [ChIP-Seq]

Chromatin alterations are important mediators of gene expression changes. We have recently shown that activated non-canonical NF-?B signaling (RelB/p52) recruits histone acetyltransferase CBP and deacetylase HDAC1 to selectively acetylate H3K9 (H3K9ac) to induce expression of corticotropin-releasing hormone (CRH) and prostaglandin-endoperoxide synthase-2 (PTGS2) in the human placenta. Both of these genes play a role in initiating. We performed chromatin immunoprecipitation followed by gene sequencing (ChIP-seq) in primary term human cytotrophoblast (CTB) with use of antibodies to RelB, CBP, HDAC1 and H3K9ac. We further associated these chromatin alterations with gene expression changes from mid-trimester to term in CTB by RNA sequencing (RNA-seq). We detected a genome-wide differential gene enrichment between mid-trimester and term human placenta. Pathway analysis identified that cytokine-cytokine receptor interaction, NF-?B, and TNF are the leading pathways enriched in term placenta and associated with these chromatin alterations. Our analysis has provided the first-time characterization of the key players of human placental origin with molecular changes resulting from chromatin modifications, which could drive human labor. Overall design: 3 individual placental specimens from full-term pregnancies were collected from healthy women with estimated gestational age (EGA) of 38 and 40 weeks who were delivered by Cesarean section (C-section). 3 individual mid-trimester placentas were collected from healthy individuals with EGA between18 to 22 weeks following elective termination of pregnancy.