RNA sequencing of preB-ALL NALM-6 cells with or without XIAP-knockdown, using prime-seq. RNA sequencing of preB-ALL NALM-6 cells with or without XIAP-knockdown, using prime-seq

In this study we performed molecular manipulation of XIAP in vivo using microRNA-30 flanked shRNA expression in cell lines models of r/r ALL. Interestingly, upon strong XIAP knockdown, r/r ALL cells were outcompeted in vivo, while intermediate knockdown sensitized cells to chemotherapy. Using prime-seq we wanted to characterized NALM-6 cells expressing different XIAP levels. Overall design: XIAP KD and control NALM-6 cells were isolated out of the murine bone marrow at different time points. the mRNA expression pattern was analyzed. NOTE FROM SUBMITTER: Due to legal regulations in Germany, we are not allowed to publish raw sequencing data. We already asked for permission to only publish the raw counts.