Differential gene expression between wild type and Pax9-null pharyngeal arch tissue

The aim of this study was to identify differentially expressed genes within the pharyngeal arches of Pax9-null embryos at E9.5. Mice null for Pax9 die in the neonatal period with complex cardiovascular defects, caused by abnormal morphogenesis of the pharyngeal arch arteries. Total RNA was isolated from dissected pharyngeal arch region of E9.5 mouse embryos. Pax9-null pharyngeal arches were compared to stage-matched wild type controls. Three biological replicates for each group (wild type vs. mutant) were collected. Samples represent a mix of female and male animals. Isolated RNA was subjected to high throughput sequencing analyses to identify differentially expressed transcripts.本研究旨在鉴定E9.5时期Pax9基因敲除（Pax9-null）小鼠胚胎咽弓（pharyngeal arches）内的差异表达基因（differentially expressed genes）。Pax9纯合缺失的小鼠会在新生期死亡，其死因是咽弓动脉（pharyngeal arch arteries）形态发生异常所导致的复杂心血管缺陷。研究人员从经解剖分离的E9.5小鼠胚胎咽弓组织中提取总RNA，将Pax9敲除型咽弓与发育阶段匹配的野生型对照样本进行比对。每组（野生型与突变型）均设置3次生物学重复样本，所有样本均混合了雌性与雄性胚胎。对提取得到的总RNA进行高通量测序分析，以鉴定差异表达转录本（differentially expressed transcripts）。