Combining Protein-Based IMAC, Peptide-Based IMAC, and MudPIT for Efficient Phosphoproteomic Analysis
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https://figshare.com/articles/dataset/Combining_Protein_Based_IMAC_Peptide_Based_IMAC_and_MudPIT_for_Efficient_Phosphoproteomic_Analysis/2952988
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资源简介：
Immobilized metal affinity chromatography (IMAC) is a common strategy
used for the enrichment of phosphopeptides from digested protein mixtures.
However, this strategy by itself is inefficient when analyzing complex
protein mixtures. Here, we assess the effectiveness of using protein-based
IMAC as a pre-enrichment step prior to peptide-based IMAC. Ultimately,
we couple the two IMAC-based enrichments and MudPIT in a quantitative
phosphoproteomic analysis of the epidermal growth factor pathway in
mammalian cells identifying 4470 unique phosphopeptides containing
4729 phosphorylation sites.
创建时间：
2016-02-27
