Martin Wuehr, Timothy J. Mitchison (2010) CIL:9512, Xenopus laevis. CIL. Dataset

Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg.

Eggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647. A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective. Lasers were 488 (DNA stain) and 633 (Tubulin stain).

非洲爪蟾（Xenopus laevis）卵母细胞处于有丝分裂的中期。请注意卵母细胞顶部左侧微小的中期纺锤体。卵母细胞经90%甲醇固定，10%（H2O 500mM EGTA）处理，并使用YoPro1（绿色）染色DNA，以及抗B-5-1-2抗体直接标记Alexa 647染色微管（红色）。采用蔡司LSM 710显微镜，使用10X 0.3NA物镜采集单个Z切片。激光分别为488nm（DNA染色）和633nm（微管染色）。