hBD3 exacerbates stimulation by the viral mimic HMW polyI:C but does not increase stimulation by other TLR ligands

(A) BMDM from 3 separate wildtype (wt) mice were incubated overnight with HKLM (Heat Killed Listeria monocytogenes) (TLR2 ligand), FSL-1 (synthetic lipoprotein Pam2CGDPKHPKSF derived from Mycoplasma salivarium) (TLR2/6 ligand), LTA (Lipoteichoic acid from S. aureus (TLR2 ligand), Pam3CSK4 (synthetic mimic of bacterial triacylated lipopeptide (TLR2/1 ligand), R848 (imidazoquinoline compound (TLR7 ligand), CpG (TLR9), TLR4 (LPS) or (B) HMW polyI:C (pI:C) (TLR3/RLR agonist) (10µg/ml) with and without hBD3. (C) PMA-differentiated THP-1 cells were treated with HMW polyI:C (10µg/ml) with or without 5 mg/ml hBD3. TNF-α, IL-6, IL-8 and CXCL10 induction were measured by ELISA *p≤0.05, **p≤0.01 student t-test (D) Human primary PBMDM were exposed to polyI:C as indicated, Interferon-β (IFNβ) gene expression was measured by qRT-PCR. ***p